Web1 day ago · We also obtained a mutant line SAIL_645_C03 (flail3) carrying a T-DNA insertion at the 3′-end of sense FLAIL locus (Figs 1A and EV1F). All three mutants reduced the expression of sense and antisense FLAIL transcripts as detected by strand-specific RT-qPCR, suggesting that they reduced the bioavailability of FLAIL isoforms (Fig 1D and E ). WebSep 19, 2024 · A T-DNA insertion line of Arabidopsis at the MORF9 locus and generation of transgenic plants. (a) Schematic of the T-DNA insertion morf9 mutant and the transgenic constructs for overexpression of a GFP-tagged MORF9 or GFP alone in wild-type (WT) plants and complementation (Pmorf9:MORF9-myc) of the T-DNA insertion morf9 mutant.

LncRNA FLAIL affects alternative splicing and represses …

WebOct 21, 2013 · In Arabidopsis, three T-DNA insertion mutant lines of MRE11 gene were previously described mre11-1, mre11-2 [ 21] and mre11-3 [ 35 ]. While mre11-1 and mre11-3 mutants were both dwarfed and sterile with many developmental defects, the mre11-2 plants displayed normal vegetative growth and fertility [ 21 ]. WebT-DNA insertion mutants of the accession Columbia (Col-0) were created by Agrobacterium tumefaciens -mediated transformation. To allow selection of transformed plants under greenhouse conditions, a sulfadiazine resistance marker was employed. hale ce story mode download

Molecular analysis of T-DNA insertion mutants identified putative ...

WebThe T-DNA sequence-indexed mutant collections contain insertional mutants for most Arabidopsis thaliana genes and have played an important role in plant biology research for almost two decades. WebNov 17, 2006 · T-DNA insertion mutants for At3g24440 were obtained from Arabidopsis Biological Resource Center (ABRC) (Alonso et al. 2003), and a homozygous line for SALK_136506 was designated as vil1-1 and SALK_140132 as vil1-2. vil1-1 and vil1-2 in Columbia were introgressed into FRI-Col (Lee and Amasino 1995). bumblebee cafe blue ridge