Webi ABSTRACT Fluorescence spectroscopy is a powerful tool for biophysical studies due to its high sensitivity and broad availability. It is possible to detect fluorescence from sing WebMar 13, 2014 · The Cy3/Cy5 chromophores were placed close to a primer/template (p/t) junction (Figure 1C) in a third class of samples. Here the donor Cy3 chromophore was attached to the 3′ end of the primer strand, while the acceptor Cy5 chromophore was incorporated into the sugar-phosphate backbone of the template strand at the ‘+1 …

Initial state of DNA-Dye complex sets the stage for protein ... - Nature

WebThe cyanine dye Cy3 is a popular fluorophore used to probe the binding of proteins to nucleic acids as well as their conformational transitions. Nucleic acids labeled only with Cy3 can often be used WebWe are able to do lifetime measurement using MEM-FLIM cameras for various objects, e.g. fluorescence plastic test slides, fluorescein solution, fixed GFP cells, and GFP - Actin … seattle children\u0027s hospital hematology

Cy3 Dyes Biocompare

WebThis is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. WebThe major advantages of plastic over aqueous mounting media are brightness, contrast, and longevity of the fluorescence. Cy2, Cy3, and Cy5 are available conjugated to a selection … WebWe offer a broad range of Alexa Fluor 568 products including protein and antibody labeling kits, secondary antibodies, bioconjugates, and more: Super-resolution microscopy (SRM) applications Antibody and protein labeling kits Streptavidin conjugates for signal amplification Tyramide labeling kits for ultimate sensitivity seattle children\u0027s hospital hematology clinic